Hello, I am Rishabh

I have a deep love for history, philosophy, art, and science, and a strong desire to understand how life works on multiple levels. I am fascinated by the molecular mechanisms of complex diseases such as cancer and metabolic disorders, as well as by fundamental biological processes. From my perspective, and as history bears witness, research is an evolving pursuit of purpose, and I continue to refine my understanding and evolve day by day. I remain open to learning, growing, and exploring new opportunities as I advance in my scientific journey.

📧 rishabhsharma200192@gmail.com

📱 +91 9625906961

Research Experience

Project Assistant

Institute of Liver and Biliary Science

Department of Transplant Hepatology

PI: Dr. Satender Pal Singh | Co-PI: Dr. Gayatri Ramakrishna

Feb 2025 - Ongoing

Project: Liquid biopsy using Circulatory Tumor DNA for predicting the Immunotherapy response in patients with Hepatocellular Carcinoma
Studying cfDNA to find Genetic and Epigenetic Biomarkers for Predicting Immunotherapy Response in HCC

Post-Graduate Research Intern

Institute of Liver and Biliary Science

Department of Cellular and Molecular Medicine

Lab: Dr. Gayatri Ramakrishna

Jan 2024 - July 2024

Study 1: Investigating the prevalence of HSD17b13 rs72613567 and PNPLA3 I148M Variant and their impact on clinical outcome in Metabolically Associated Fatty Liver Disease in the North Indian community
Collected blood samples, conducted genotyping using PCR-RFLP and performed data analysis
Study 2: Association of PNPLA3 I148M Variant and Leukocyte Telomere Length with Alcohol-Induced Liver Disease: A hospital and community-based study
Conducted RT-PCR and assisted with data analysis

Summer Research Intern

Translational Oncology Department, Hansraj College

Delhi University | Lab: Dr. Tejveer Singh

July 2023 - Aug 2023

Project: Exploring prominent avenues in CTC research and the role of CTCs as a biomarker in Head and neck cancer

M.Sc. Dissertation

Institute of Liver & Biliary Sciences

Under supervision of Dr. Gayatri Ramakrishna

2023-2024

Title: Investigation of the Prevalence and Impact of PNPLA3 rs738409 on Clinical Outcomes in Alcoholic Liver Disease and the Community in North Indians

Publications

Cell-Free DNA in Hepatocellular Carcinoma: From Biology to Treatment Response

Rishabh Sharma, Satender Pal Singh, Garima Bhatia, and Gayatri Ramakrishna World Journal of Hepatology. 2025.

Under Review

Molecularly Distinct TP53-Driven and Oncogene-Addicted Genetic Clusters in Indian Gallbladder Cancer and Insights into Genetic Variation Patterns Linked to Gallstone Status

Vipin Yadav, Archana Rastogi, Vaishali Yadav, Ashily Koshi, Abhilasha Yadav, Rishabh Sharma, Ragini Kilambi, Nihar Mohapatra, Nirupama Trehanpati, Viniyendra Pamecha, Gayatri Ramakrishna, and Shiv K. Sarin

Under Review

Artificial blood-hope and the challenges to combat tumor hypoxia for anti-cancer therapy

Rishabh Sharma, Manju Kashyap, Hatem Zayed, Lucky Krishnia, Manoj Kumar Kashyap

Med Biol Eng Comput. 2025 Apr;63(4):933-957. doi: 10.1007/s11517-024-03233-6

Emerging Trends in Neuroblastoma Diagnosis, Therapeutics, and Research

Rishabh Sharma, Jaya Yadav, Deepika Sharma, Manju Saini, Nipun Padha, Sajad Ahmad Bhat, Abdugani Musayev, Tejveer Singh

Molecular Neurobiology. 2025 May;62(5):6423-6466. doi: 10.1007/s12035-024-04680-w

The Role of Phytonutrient Kaempferol in the Prevention of Gastrointestinal Cancers: Recent Trends and Future Perspectives

Tejveer Singh, Deepika Sharma, Rishabh Sharma, Hardeep Singh Tuli, Shafiul Haque, Seema Ramniwas, Darin Mansor Mathkor, Vikas Yadav

Cancers (Basel). 16(9):1711 doi:10.3390/cancers16091711

Is the Liver Resilient to the Process of Ageing?

Nirupama Chatterjee,Rishabh Sharma, Pratibha R Kale, Nirupma Trehanpati, Gayatri Ramakrishna

Annals of Hepatology 101580. 2024. doi: 10.1016/j.aohep.2024.101580

Diagnostic Performance of Circulating Cell-Free DNA 5-Hydroxymethylcytosine Signatures for Hepatocellular Carcinoma: Meta-Analysis

Rishabh Sharma, Satender Pal Singh, Eduardo Solano Pina dos Santos

Under Review, 2025. Preprint available at SSRN: https://ssrn.com/abstract=5381270

Gender Differences in Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD): Epidemiology, Pathophysiology, and Clinical Implications

Kaushal K, Singh S, Anand A, Ramakrishna G, Kale P, Rishabh Sharma, Rewari BB, Sarin SK

Journal of Clinical and Experimental Hepatology. Under Review, 2025

Transjugular liver biopsy is safe and yields adequate tissue in acute on chronic liver failure and chronic liver disease

Satender Pal Singh, Rajan Vijayaraghavan, Harshvardhan Tevethia, et al.

Liver International. Under Review, 2025

Radiocarbon dating and multi-omics of gallstones reveal prolonged residence time accompanied with microbial dysbiosis and bacterial oncometabolites linked to gallbladder cancer.

Vipin Yadav, Rajveer Sharma, Ashi Mittal, Garima Bhatia, Vaishali Yadav, Rishabh Sharma, Ragini Kilambi, Deepti Sharma, Ankita Patel, Nihar Mohapatra, Pratibha Kale, Sherin Thomas, Archana Rastogi, Nirupma Trehanpati, Vinniyendra Pamecha, Shvetank Sharma, Shiv Kumar Sarin, Gayatri Ramakrishna.

JHEP Reports, (Under Review)

Published Abstracts / Conference Proceedings

PNPLA3 RS738409 Risk Variant Outperforms MBOAT7 RS641738 Polymorphism in Predicting Alcohol Related Liver Disease (ALD) and Short Leukocyte Telomere Length Is Associated with High FIB4 Score

Vaishali Yadav, Saggere Muralikrishna Shasthry, Kanica Kaushal, Mohit Varshney, Satender Singh, Rishabh Sharma, Guresh Kumar, Nirupama Chatterjee, Nirupma Trehanpati, Shiv Kumar Sarin

Hepatology. 2025; 82(S1): S1–S2308. [Abstract #1608]. The Liver Meeting 2025 (American Association for the Study of Liver Diseases, AASLD). DOI: 10.1097/HEP.0000000000001493

Alcohol-induced liver disease is associated with PNPLA3 I148M (rs738409) genetic variant and shorter leukocyte telomere length: A hospital and community-based study

Yadav Vaishali, Shasthry Saggere Muralikrishna, Kaushal Kanica, Varshney Mohit, Chatterjee Nirupama, Singh Satender, Rishabh Sharma , Trehanpati Nirupma, Ramakrishna Gayatri.

Indian Journal of Gastroenterology. 2024; 43(Suppl 1): 1–184. The 65th Annual Conference of the Indian Society of Gastroenterology (ISGCON 2024), Varanasi, India, December 4–7, 2024. DOI: 10.1007/s12664-024-01700-4

Two distinct genetic subtypes define non-cirrhotic HCC characterized by inactivation of tumor suppressors (TP53, RB1, ARID1A) and activation of the TERT-driven oncogenic pathway.

Agrawal Khushboo, Rastogi Archana, Gautam Pramod, Yadav Vaishali, Rishabh Sharma, Garg Vanya, Sharma Deepti, Trehanpati Nirupma, Sarin Shiv Kumar.

Hepatology 2025; 82(S1): S4282. The Liver Meeting 2025 (American Association for the Study of Liver Diseases, AASLD). DOI: 10.1097/HEP.0000000000001493

Additional Work Experience

Science Teacher

Alphonse Public School, Harit Vihar, Sant Nagar

Taught science subjects to middle and high school students, developed curriculum materials, and mentored students in science projects.

Mar 2018 - Sep 2022

Telecaller Executive

Eastbound Venture Pvt Ltd, New Delhi

Handled customer communications, maintained databases, and assisted in client relationship management.

Sep 2016 - Jan 2018

Workshops & Conferences

Poster Presentation - Asian Pacific Digestive Week

Abstract titled "Diagnostic Performance of Circulating Cell-Free DNA 5-Hydroxymethylcytosine Signatures" accepted for presentation at this prestigious international conference.

2025

Hands-on Workshop on Liquid Biopsy

14th Dr. V. Ramalingaswami Oration & Liquid Biopsy Workshop conducted by the Department of Pathology, ILBS. Gained practical experience in cfDNA and 16s library preparation techniques.

2025

Hands-on Workshop on Rodent Surgery

Comprehensive training in rodent handling, surgical procedures, and animal care conducted by the Institute of Liver and Biliary Sciences.

2024

Certifications & Diplomas

🎓

Understanding Cancer Metastasis

Johns Hopkins University

2022

🧬

Certificate Course in Immunology

Indian Institute of Technology Kharagpur

2021

🧠

Introduction to Brain & Behavior

Indian Institute of Technology Kharagpur

2021

🇯🇵

Certification Course in Japanese Language

Indira Gandhi National Open University

2018-2021

🇩🇪

Certificate Course in German Language

Amity University Gurugram

2022-2024

💻

Advanced Diploma in Web Designing

Adarsh Technical Institute, Mukundpur, New Delhi

2014

Achievements & Extra-Curricular Activities

🏆

First Position - Interschool Singing Competition

Won first place in interschool singing competition at Rani Public School, 2015

🥈

Second Position - Orchestra Competition (3 Consecutive Years)

Secured second position for three consecutive years at zonal level competition organized by Delhi Sports and Cultural Activities, 2013-2016

🎵

Semi-Classical Singing Awards

Achieved second and third positions in semi-classical singing at zonal and district levels in competitions organized by Delhi Sports and Cultural Activities, 2012

🤝

Community Service Volunteer

Actively volunteered for community-based screening and cancer awareness programs at ILBS, 2024

🌍

International Equanimity Olympiad Participant

Participated in the International Equanimity Olympiad, 2015

Professional References

Dr. Gayatri Ramakrishnan

Professor
Department of Molecular and Cellular Medicines
Institute of Liver and Biliary Science
New Delhi, India

📧 rgayatri@ilbs.in

📱 +91-9540946978

Dr. Manoj Kumar Kashyap

Associate Professor
Amity Institute of Stem Cells
Amity University Haryana
Haryana, India

📧 mkkashayap@ggn.amity.edu

📱 +91-9759221001

Dr. Tejveer Singh

Assistant Professor
Translational Oncology Lab
Department of Zoology, Hansraj College
University of Delhi, New Delhi, India

📧 tejveer@hrc.du.ac.in

📱 +91 98872 97586

Technical Skills

🧬 Molecular Biology

Real-time PCR
PCR-RFLP
Gel electrophoresis
cfDNA isolation from plasma
RNA isolation (Trizol method)
Genomic DNA isolation
Metabolomics & Proteomics sample prep

🔬 Cell Biology

PBMCs isolation
Granulocyte isolation
Mammalian cell culture
Flow cytometry
ELISA
IHC & Immunofluorescence
Ascitic fluid cell isolation

💻 Bioinformatics

Multi-Omics data analysis
Protein visualization (PyMOL, Chimera)
Phylogenetic analysis (MegaX)
Molecular docking
Primer designing
Single cell data analysis
R programming & Python

🐭 Animal Work

Rodent handling
IP, IM, Subcutaneous injections
Oral administration
Blood withdrawal (tail & retro-orbital)
Survival surgery
Colon ligation
Necropsy & Diet preparation

📊 Software & Programming

SPSS Statistical Analysis
R Programming
Python (Fundamental)
Microsoft Excel (Advanced)
Data Visualization

🌍 Languages

Hindi (Native)
English (Fluent)
Spanish (A1)
German (A1)